Boca virus nucleic acid detection kit (PCR-fluorescent probe method)
【Clinical manifestations】
Bocavirus is a genus of the Parvoviridae subfamily of the Parvoviridae, first discovered in animals in the early 1960s. In August 2005, Swedish scientists used molecular virus screening methods for the first time to discover a new type of human parvovirus in children's respiratory secretions, named human Boca virus, which is closely related to human acute respiratory infections, and the symptoms are common Colds are similar. The same virus was found in Australia and China, and the infection rate was greater than 5.6%.
【Features】
This kit uses real-time fluorescent PCR technology, takes the highly conserved region of the Boca virus genome as the target region, designs specific primers and fluorescent probes, and then performs PCR amplification on the target sequence, while monitoring the amplification in real time by Taqman fluorescent probe technology The accumulation of products enables qualitative detection of Norovirus nucleic acids in the sample. The reaction system of this kit contains dU-UNG enzyme anti-pollution system to avoid false positive results.
【Product Performance】
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Sensitivity
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1.0x103 copies / mL
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Linear range
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1.0x103 copies / mL ~ 1.0x 109 copies / mL
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Accuracy
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The detection result coincidence rate is 100%
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Precision
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Coefficient of variation within and between batches CV≤5%
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Specificity
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100% specificity, no cross reaction with influenza A virus, influenza B virus, adenovirus , human metapneumovirus, rhinovirus, Epstein-Barr virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, etc.
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Anti-interference
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Oxymetazoline hydrochloride sprays, budesonide nasal spray, azithromycin neomycin, zanamivir, ribavirin and the like without significant interference to the detection result
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【Product information】
Test specimen: throat swab
Technical principle: PCR- fluorescent probe method
Packaging specifications: 24 tests/ kit, 48 tests/ kit, 96 tests/ kit
Applicable instruments: Roche Cobas z 480; Roche LightCycler 480; ABI 7500; Shanghai Hongshi SLAN-96S; Bioer LineGene 9600, etc.
